Protein Solubilization, Protein Renaturation Resources

OptiSol FAQ (Frequently Asked Questions about the OptiSol Protein Solubility Screens)

Request Brochure or Manual for the OptiSol Protein Solubility Screening Kit

Introduction to the basic concept of Protein Solubility and Protein Solubilization.

Recent Blog posts: A protein purification classic: Ammonium sulfate precipitation, an underutilized way to purify proteins: Ammonium sulfate precipitation

Online tools for Protein Solubility prediction starting from amino acid sequence

Prediction of Protein Solubility Recombinant Protein Solubility prediction from Univ. Oklahoma, School of Chemical Engineering and Materials Science. Very simple to use.
SOLPro predicts the propensity of a protein to be soluble upon overexpression in E. coli using a 2-stage SVM architecture based on multiple representations of the primary amino acid sequence. From the Univ. California Irvine, Inst. of Genomics and Bioinformatics
PROSO for multiple, alternative proteins tries to answer this question: "Which of my cloned proteins have the best/worst chances to be soluble upon heterologous expression?" From the Helmholz Zentrum Munich, Institute for Bioinformatics and Systems Biology

Online tools for Protein Folding prediction starting from amino acid sequence

FoldIndex: Will this Protein Fold? Useful to assess chances of protein renaturation and protein refolding experiments. From the Weitzman Institute, Israel
FoldUnfold predicts disordered regions in a peptide chain.
REFOLD database lists refolding conditions for more than 300 E.coli proteins. The rationale and use of the database are described in this article.

Protocols on Protein Renaturation and Protein Refolding

Excellent article on Protein Precipitation in Wikipedia.

Selected References on the topic of Protein Solubility:

pH and buffer type effecting protein solubility

Chan, P., Warwicker, J. (2009) Evidence for the adaptation of protein pH-dependence to subcellular pH. BMC Biology, 7:69.

Jancarik et al. (2004). Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins. Acta Cryst. D 60:1670.

Increased solubility by 'salting in' of protein solutions

Jenkins, W.T. (1998). Three solutions of the protein solubility problem. Protein Sci. 7(2):376-82.

Certain additives shown to enhance the solubility of proteins

Arakawa et al., 2006 & 2007. Arakawa,T., Philo,J.S., Ejima,D., Tsumoto,K., Sato,H., Arisaka, F. (2006 & 2007) Aggregation analysis of therapeutic proteins; parts 1, 2, 3. Bioprocess International 4 (10) : 32-42; 5 (4) : 36-47; 5 (10) : 52-70.

for instance, dramatic increase in long-term stability of protein samples by combining Arginine and Glutamate.

Golovanov AP, Hautbergue GM, Wilson SA, Lian LY. (2004). A simple method for improving protein solubility and long-term stability. J Am Chem Soc. 126(29):8933-9.

Manuals

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