How Protein Solubility Optimization with OptiSol works:

OptiSol is based on Dilyx’ proprietary FASOS Technology. FASOS stands for Filter Aided Solubility Optimization Screening.  A proteins solution is mixed separately with many different solutions and only those protein solutions that pass a Molecular Weight Cutoff Filter (MWCO 30 or MWCO 200) are detected. This combinatorial solution and filtration approach identifies the optimal pH, salt, buffer type, additives and antioxidants that maintain samples solubilized. Either solubilize an aggregated protein solution (Application A) or select a particular sample stress (heat, freeze-thaw, storage, shear etc.) and identify those solutions that keep your protein sample solubilized without aggregation. Data evaluation is supported with the Protein Dashboard. To see what your colleagues say about OptiSol click here.

Application A:  Protein Solubility Profiling

Application B: Protein Aggregate Solubilization

Compositions of OptiSol Solutions

OptiSol Manual

Application A: Protein Solubility Profiling

OptiSol reagents   

Combinatorial solution set in 96-well format with a variety of pH, buffer types, charged & neutral organic additives and antioxidants. Details are available online*.

Sample stress   

Choose the stress that is known or suspected to trigger aggregation, such as heat, storage, freeze/thaw, agitation or shear.

Filter plate   

Available for proteins smaller than 30 kDa or smaller than 200 kDa.

Suitable assays  

Specific assays work best. Functional assays, biophysical assays, or when purified protein samples are used, simple protein quantification assays are suitable.

Protein Dashboard  

Excel-based spreadsheet for automated graphing and quick evaluation; comes with OptiSol kit.

Application B: Protein Aggregate Solubilization

Protein Aggregate    

Reversibly aggregated protein samples can be broken up if the proper aqueous milieu is provided. Under optimized conditions the particles pass the MWCO filter as individual units.

Filter plate   

Available for proteins smaller than 30 kDa or smaller than 200 kDa.

Suitable assays    

Specific assays work best. Functional assays, biophysical assays, or when purified protein samples are used, simple protein quantification assays are suitable.

Protein Dashboard   

Excel-based spreadsheet for automated graphing and quick evaluation; comes with OptiSol kit.