OptiResc™

Protein Aggregate Solubilization Kit

Proteins aggregate for a variety of reasons. Fortunately, reversibly aggregated protein samples can be brought back into solution by adjusting pH, ionic strength and additive content. This OptiResc Protein Aggregate Solubilization kit helps identifying the solution parameters that dissolve reversibly aggregated protein.

'Crashed out' protein sample?

Use OptiResc to bring it back into solution.

Just mix and observe.

Proteins aggregate. This undesired process is often encountered when changing buffers, after proteolytic cleavage of an epitope tag or just over a period of time. Reversibly aggregated protein solutions can be 'brought back' into solution by dissolving them into the correct buffer (see this video).

This OptiResc Protein Aggregate Solubilization Kit contains a systematically varied array of buffers (from pH 3 to pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that help to determination conditions under which a particular aggregated protein sample dissolves.

Protocol: Identify Conditions that Solubilize Reversibly Aggregated Protein Samples:

Material: aggregated protein sample (ca. 50 uL total for each protein sample to be tested) – up to six different protein samples can be applied with one kit (3 Inspection Plates with 2 x 96 OptiResc experimental wells).

Protocol for two aggregated protein samples:

1.Distribute 50 uL aliquots from wells from OptiResc Reagent plate wells into large reservoir wells of OptiResc Inspection Plate (all 96 wells).

2.Transfer 2 x 0.5 uL from each reservoir well of OptiResc Inspection Plate into small wells of OptiResc Inspection plate.

3.Add 0.5 uL of aggregated protein sample A to each OptiResc experimental well (upper small well A) and add sample B to each OptiResc experimental well (lower well B) within the OptiResc Inspection Plate. Omit sample in position H1 (negative control).

4.Seal OptiResc Inspection Plate with one Seal Sheet.

5.Inspect small wells with a microscope, i.e. a dissecting stereo microscope and note the wells that contain precipitate (aggregated protein solution) or clear drops (dissolved protein). If no clear drops are seen, inspect again after 1 hour or 1 day.

6. Use the OptiResc Reagent Listing to identify solutions that yield clear drops.

7. Scale up disaggregation reaction by mixing equal volumes of aggregated protein sample with solution that was identified with the OptiResc kit.

OptiResc kit Features:

• Quickly evaluate solubilizaiton behavior as a function of pH, salt and additives

• Assay more than 90 different conditions in parallel and label-free

• Simple mix and observe with a microscope, no furhter handling required

No more aggregated protein. Order here FAQ Tell me more.

Get in touch

Email info@dilyx.com

→ Inquiry form

Place an order

→ pricing information

Interested in working for us?

→ Drop us a note