Dilyx OptiSol™ Protein Solubility Screening Kit
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Proteins and Peptides behave well in a micro environment that fits their needs. Some proteins are "happy" only in acidic buffers, others require high salt and a metabolite to stay in solution. What do you know about the preferences of your protein target?
Find your protein's solubility sweet spot with the OptiSol kit
Proteins aggregate. This undesired process is often caused by elevated temperature, vigorous stirring, addition of ligands or molecular binding partners or just by mere storage of a protein sample over a period of time. Protein aggregation can often be avoided with proper choice of pH, salt or stabilizing additives. This OptiSol Protein Solubilization Screening Kit contains a systematically varied array of buffers (from pH 3 to pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that enable the determination of conditions under which a particular protein sample is protected from aggregation or can be de-aggregated. A total of 90 different formulations with solubility enhancers can be tested in one single, label-free experiment; the remaining 6 experiments are positive and negative control experiments.
Soluble proteins pass filters, aggregated proteins don’t. This simple feature is exploited to in the filtration step. The wells that contain soluble proteins will yield protein after the filtration process, those with protein aggregates will not contain any protein.
The OptiSol Protein Solubility Screening Kit employs a systematic solution design and array-based filtration technology that enables you to either
→ identify formulations that protect your target protein from aggregation, or to
→ solubilize an aggregated protein sample.
This is how the OptiSol kit works:
Protein Solubility Profiling |
Solubilize Aggregated Protein |
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The accompanying Protein Dashboard™ aids in spotting protein behavior trends and identify the critical solvent factors for optimal protein solubilization.
This is what your colleagues say about the OptiSol kit (more user feedback here).
“I think the Optisol Kit is a really great idea…”; “The kit worked very well.”
"I was not expecting much but the assay worked much better than I expected. I noticed many trends that may improve the solubility of my pellet.”
“It is a great and unique opportunity to quickly assess the optimal conditions of your favorite protein.”
“The ProteinDashboard was very informative”
OptiSol kit Features:
* The OptiSol kit can be used to optimize your protein's solubility and withstand stresses caused by elevated temperature, long-term storage, ice/liquid transition (freeze/thaw), shear forces, intense light, surface exposure, air or chemical oxidation.
After running the Protein Solubilization Kit you have established conditions that keep your protein target in solution and you know conditions at which your protein aggregates. Then choose conditions to obtain a "well behaved protein".
- Better and faster than a conventional trial-and-error testing (less than 1 h)
- Low protein consumption consumes less than 10 uL for each protein solubility data point. The protein quantity needs to be matched with your target detection assay
- Convenient: get a lot of information on protein solubilization in one go. Utilize the target specific assay of your own choice. The OptiSol Protein Solubility Screening kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry and fluorescence-based protein detection.
No more aggregated protein. Order here FAQ Tell me more.
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Email info@dilyx.com |
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