Protein Solubilization Kit

Proteins and Peptides behave well in a micro environment that fits their needs. Some proteins are "happy" only in acidic buffers, others require high salt and a metabolite to stay in solution. What do you know about the preferences of your protein target?

Find your protein's solubility sweet spot with the OptiSol kit

The OptiSol Protein Solubility Screening Kit employs a systematic solution design and array-based filtration technology that enables you to either
→ identify formulations that protect your target protein from aggregation, or to
→ solubilize an aggregated protein sample.

The accompanying Protein Dashboard™ aids in spotting protein behavior trends and identify the critical solvent factors for optimal protein solubilization.

Features:
• Quickly evaluate aggregation behavior as a function of pH, salt and additives
• Assay more than 90 different conditions in parallel and label-free
• Simple mix and spin protocol, no ultracentrifugation required
• Opportunity to rescue reversibly aggregated protein samples
• Protect your protein from aggregation under stress*

* The OptiSol kit can be used to optimize your protein's solubility and withstand stresses caused by elevated temperature, long-term storage, ice/liquid transition (freeze/thaw), shear forces, intense light, surface exposure, air or chemical oxidation.

After running the Protein Solubilization Kit you have established conditions that keep your protein target in solution and you know conditions at which your protein aggregates. Then choose conditions to obtain a "well behaved protein".

Better and faster than a conventional trial-and-error testing (less than 1 h)
Low protein consumption consumes less than 10 uL for each protein solubility data point. The protein quantity needs to be matched with your target detection assay
Convenient: get a lot of information on protein solubilization in one go. Utilize the target specific assay of your own choice. The OptiSol Protein Solubility Screening kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry and fluorescence-based protein detection.

No more protein aggregates.